pb2 protein virus

Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.

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This section provides any useful information about the protein, mostly biological knowledge.

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Manually curated information for which there is published experimental evidence.

For influenza A viruses (IAVs), a composite pattern of PTMs regulates the activity of viral proteins. S5A and S5B). This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.

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The Gene Ontology (GO) project provides a set of hierarchical controlled vocabulary split into 3 categories:

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UniProtKB Keywords constitute a controlled vocabulary with a hierarchical structure. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. only protein content? The viral polymerase basic protein 2 (PB2) plays an impor-tant role in the adaptation and pathogenesis of avian influ-enza virus in mammalian hosts (Subbarao et al., 1993; Hatta et al., 2001; Gabriel et al., 2005; Li et al., 2005; Min et al., 2013).

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) BarclaySequences in influenza A virus PB2 protein that determine productive infection for an avian influenza virus in mouse and human cell lines J. UR - http://www.scopus.com/inward/record.url?scp=85069610225&partnerID=8YFLogxK, UR - http://www.scopus.com/inward/citedby.url?scp=85069610225&partnerID=8YFLogxK, Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2020 Elsevier B.V, "We use cookies to help provide and enhance our service and tailor content. It is also a substrate for P-glycoprotein and OATP1B1 and also inhibits P-glycoprotein.

Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. View Product. Pimodivir 600 mg twice daily for 5 days has been selected and is undergoing further evaluation in phase III clinical trials.".

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This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.

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This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.

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This subsection of the Names and taxonomy section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.

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This subsection of the Names and taxonomy section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. PubMed:22090209: PB1: Influenza A_PB1_replication-efficiency_473(2)_473L, 598L_Decreased-replication-efficiency: 473L, 598L: Yes from the sequence.

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Manual validated information which has been generated by the UniProtKB automatic annotation system.

PB2, PB1, and PA subunits protein composes the influenza A virus viral polymerase complex, which is used to replicate and transcribe the viral genome in the cell nucleus. Dive into the research topics of 'Polymerase basic protein 2 (PB2) (influenza virus) inhibitor Treatment of influenza A infection'. Yellow: WGA life stained at 37ºC, 30 min. Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. N2 - Pimodivir (VX-787, JNJ-63623872) is an investigational anti-influenza drug, currently undergoing clinical development. Green: PB2 protein stained by Influenza A Virus PB2 antibody (GTX125926) diluted at 1:500. matt 2020-06-29T21:05:19+00:00. Specific drug interactions have not yet been studied. Specific drug interactions have not yet been studied. Please consider upgrading,

An evidence describes the source of an annotation, e.g. This isoform has been chosen as the It possesses two nuclear localization signals that The main difference is the immunogen used to make them. The replication of the PB2 627K mutant virus was also unchanged in this context. Pimodivir is a substrate for CYP3A4 metabolism but itself has no inhibitory or induction effects on the isoenzyme. Green: PB2 protein stained by Influenza A Virus PB2 antibody (GTX125925) diluted at 1:500. Most human seasonal influenza virus pre-2009 H1N1, H2N2, and H3N2 strains encode mitochondrial PB2 with asparagine at position 9 (N9-PB2). These are stable identifiers and should be used to cite UniProtKB entries. As of 2005, 75% of H5N1 human virus isolates from Vietnam had a mutation consisting of Lysine at residue 627 in the PB2 protein; which is believed to cause high levels of virulence. This complex is responsible for the synthesis of both genomic and messenger viral RNA, the hereditary and infective agents of the flu virus. It is also a substrate for P-glycoprotein and OATP1B1 and also inhibits P-glycoprotein. Sometimes however, changes in the PB2 protein allow bird flu viruses to better replicate in humans, potentially leading to deadly flu pandemics. Bake. Peanut Butter Oatmeal Cookies Recipe. Influenzavirus A PB2 protein Medicine & Life Sciences VX-787 Medicine & Life Sciences Polymerase basic protein 2 (PB2) (influenza virus) inhibitor Treatment of influenza A infection. These elements correspond to the DSSP secondary structure code 'T'.

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This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

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This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.

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This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.

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This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. T1 - Polymerase basic protein 2 (PB2) (influenza virus) inhibitor Treatment of influenza A infection.

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When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later.

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This indicates the type of evidence that supports the existence of the protein. It is potent against influenza A, including against adamantanes and neuraminidase inhibitor-resistant strains. These findings imply that huANP32A has less of a role in maintaining the high polymerase activity of H7N9 PB2 627K virus. The single amino acid substitution at position 627 from Glu to Lys (E627K) in PB2 of avian-origin influenza virus was first revealed to be responsible for more efficient replication of the virus in mammalian cells (22). The current subsections and their content are listed below:

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This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

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This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Interestingly, multiple mouse passages of the r/NS1HL17 and r/NS1HL18 viruses resulted in selection of mutant viruses containing single amino acid mutations in the viral PB2 protein. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

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Interacts with nucleoprotein NP (via N-terminus). Manual assertion based on experiment ini, Reactome - a knowledgebase of biological pathways and processes, ChEMBL database of bioactive drug-like small molecules. You are using a version of browser that may not display all the features of this website. Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response. Research output: Contribution to journal › Article › peer-review. PB2 Performance Almond Protein. Pimodivir is a substrate for CYP3A4 metabolism but itself has no inhibitory or induction effects on the isoenzyme.
ATP Binding Cassette Transporter, Subfamily B, Member 1, Polymerase basic protein 2 (PB2) (influenza virus) inhibitor Treatment of influenza A infection. The influenza A virus replicates in a broad range of avian and mammalian species by hijacking cellular factors and processes. PB2 codes for the PB2 protein which is a critical component of the viral polymerase.

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Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.

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This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). This is known as the 'taxonomic identifier' or 'taxid'.

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This subsection of the Names and taxonomy section contains the taxonomic hierarchical classification lineage of the source organism. Of note, PB2 292V and M1 156D appear in most H7N9 viruses, and the PB2 627K mutation could easily occur when the H7N9 virus replicates in humans. All positional information in this entry refers to it. In a phase IIb study of naturally acquired influenza A virus infection, a statistically significant and dose-dependent decrease in virus load was observed. Diarrhea was the most common adverse event reported and was typically mild. PB2 Protein from influenza virus H1N1: PB2 is one of four proteins which make up the influenza A virus replication complex. Pimodivir is a substrate for CYP3A4 metabolism but itself has no inhibitory or induction effects on the isoenzyme. The information is filed in different subsections. It is updated at every UniProt release.

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This section provides information on the tertiary and secondary structure of a protein.

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This subsection of the 'Structure' section is used to indicate the positions of experimentally determined helical regions within the protein sequence.

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Manually validated information inferred from a combination of experimental and computational evidence.

However, the molecular mechanisms involved remain unknown. Pimodivir 600 mg twice daily for 5 days has been selected and is undergoing further evaluation in phase III clinical trials. PB2-627K and PB2-701N enhanced viral virulence in …

However UniProtKB may contain entries with identical sequences in case pCA-PB2-S1 was generated by using a PCR-based standard technique based on pPolI-PB2. doi = "10.1358/dof.2019.44.5.2978057", https://doi.org/10.1358/dof.2019.44.5.2978057. ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. PB2-E627V has been recently shown to increase H5N1 virus replication in human cells . Pimodivir is a non-nucleoside analogue that targets the cap-binding activity of the influenza A virus PB2 polymerase subunit. It is useful for tracking sequence updates.

@article{457aa7b37a3a401f90e8405e0cc2439e. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Supportively, in H5N6-infected DF-1 cells, endogenous CCT5 is also shown to interact with viral NP and PB2 proteins (Figures 3C, D). Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. / Berg, M.; Temesgen, Zelalem. The algorithm is described in the ISO 3309 standard.

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, Manual assertion inferred from combination of experimental and computational evidence,

This subsection of the 'Structure' section is used to indicate the positions of experimentally determined beta strands within the protein sequence.

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This subsection of the 'Structure' section is used to indicate the positions of experimentally determined hydrogen-bonded turns within the protein sequence. Diarrhea was the most common adverse event reported and was typically mild.

Manually curated information which has been propagated from a related experimentally characterized protein.

Avian influenza A viruses (AIVs) generally propagated poorly in mammalian cells, but some mutants of virus-encoded RNA polymerase components, especially PB2 subunit, can overcome host restriction. Sequence conflicts are usually of unknown origin.

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This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).

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This section is used to point to information related to entries and found in data collections other than UniProtKB.

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This section provides general information on the entry.

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This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.

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A UniProt proteome can consist of several components.

The component name refers to the genomic component encoding a set of proteins.

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This section provides information on the location and the topology of the mature protein in the cell.

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This section provides information on the disease(s) and phenotype(s) associated with a protein.

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This section describes post-translational modifications (PTMs) and/or processing events.

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This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.

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This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.

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This subsection of the 'Interaction' section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the 'Function' section).

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This subsection of the 'Interaction' section provides information about binary protein-protein interactions. We identified a naturally occurring amino acid mutation in the PB2 protein of H1N1 SIVs that impacts the viral replication and virulence in mice by altering the viral polymerase activity. Peanut … FAQs for Influenza A Virus PB2 Antibody (NBP2-42878). This is also the sequence that appears in the downloadable versions of the entry. By continuing you agree to the use of cookies. 5410-5415

What is the canonical sequence?

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canonicali sequence. Yellow: WGA life stained at 37ºC, 30 min. Diarrhea was the most common adverse event reported and was typically mild. Almond Protein PB2. of multiple genes (paralogs).

The activity of PG/S1421(H7N9) polymerase bearing the PB2 627K mutation was not affected by the depletion of ANP32A protein in human cells. It binds to the cap structure at the 5' end of host mRNA to generate short capped RNA fragments that are used as primers for viral transcription named cap-snatching. In contrast to the parental viruses, virulence and IFN antagonism were restored in the selected PB2 mutants. The PB2 protein of the influenza virus RNA polymerase is a major virulence determinant of influenza viruses. Peanut Protein PB2. Influenza A virus causes influenza in birds and some mammals, and is the only species of the genus Alphainfluenzavirus of the virus family Orthomyxoviridae. Virol., 75 (11) (2001), pp. Specific drug interactions have not yet been studied. author = "M. Berg and Zelalem Temesgen".

In a phase IIb study of naturally acquired influenza A virus infection, a statistically significant and dose-dependent decrease in virus load was observed.

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Introduction of Val473Leu and Pro598Leu substitutions in the recombinant virus A/Cambodia/P0322095/2005 (PB1, PB2, PA, NP) x WSN conferred decreased polymerase activity in 293 Tcells. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the IntAct database. Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018. Pimodivir (VX-787, JNJ-63623872) is an investigational anti-influenza drug, currently undergoing clinical development. This invention also relates to computational methods using the structural coordinates of said complex to screen for and design compounds that interact with the RNA cap binding pocket.

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This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. It is potent against influenza A, including against adamantanes and neuraminidase inhibitor-resistant strains. I am a peanut butter addict. WHAT PEOPLE ARE SAYING “PB2 might be the best thing to ever happen to me. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.

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This subsection of the 'Entry information' section provides one or more accession number(s). keywords = "Influenza A, JNJ-63623872, Pimodivir, Polymerase basic protein 2 (PB2), VX-787". This section is only present in reviewed entries, i.e.

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Manually curated information for which there is published experimental evidence.

These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Blue: DAPI staining. Because PB2 proteins form part of the viral polymerase (the structure that copies the viral genome), this prevents bird flu viruses from replicating efficiently in humans. The virus carrying PB2-D701N replicated better than did wild-type Anhui/1 virus, which possesses PB2-627K, in both human and avian cells. title = "Polymerase basic protein 2 (PB2) (influenza virus) inhibitor Treatment of influenza A infection". Pimodivir is a non-nucleoside analogue that targets the cap-binding activity of the influenza A virus PB2 polymerase subunit. Protein interaction database and analysis system, BindingDB database of measured binding affinities,

Manually validated information inferred from a combination of experimental and computational evidence.

In this study, we found two amino acids in PB2 (292V and 627K) and one in M1 (156D) that are extremely important for H7N9 virus transmission. Manual assertion inferred from combination of experimental and computational evidencei, Manual assertion inferred from combination of experimental and computational evidencei, SWISS-MODEL Repository - a database of annotated 3D protein structure models, Database of comparative protein structure models, Protein Data Bank in Europe - Knowledge Base, Relative evolutionary importance of amino acids within a protein sequence, Gene3D Structural and Functional Annotation of Protein Families, Integrated resource of protein families, domains and functional sites, Superfamily database of structural and functional annotation. Gallery Peanut Butter Oatmeal Cookies Recipe . The PB2 subunit of the influenza virus RNA polymerase is a major virulence determinant of influenza viruses. Mingay, J.W. Together they form a unique fingerprint. It is also a substrate for P-glycoprotein and OATP1B1 and also inhibits P-glycoprotein. The multifunctional nature of viral proteins is essentially driven by posttranslational modifications (PTMs) and is key for the successful outcome of infection. Specific drug interactions have not yet been studied. In line with this conclusion, the PB2 protein of the H5N6 virus could be pulled down by CCT5 and vice versa (Figure 3B). Fingerprint Dive into the research topics of 'Polymerase basic protein 2 (PB2) (influenza virus) inhibitor Treatment of influenza A infection'. We'd like to inform you that we have updated our Privacy Notice to comply Pimodivir is a substrate for CYP3A4 metabolism but itself has no inhibitory or induction effects on the isoenzyme. Influenza A Virus PB2 antibody detects PB2 protein at by immunofluorescent analysis.

What is the canonical sequence?

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The checksum is a form of redundancy check that is calculated TRIM35-induced K48-linked polyubiquitination also led to the degradation of the PB2 protein of AH05 (H5N1) and AH13 (H7N9) virus (Fig. In a phase IIb study of naturally acquired influenza A virus infection, a statistically significant and dose-dependent decrease in virus load was observed. Manual assertion inferred from sequence similarity toi, DNA Data Bank of Japan; a nucleotide sequence database, Database of genes from NCBI RefSeq genomes, KEGG: Kyoto Encyclopedia of Genes and Genomes, ProtoNet; Automatic hierarchical classification of proteins, MobiDB: a database of protein disorder and mobility annotations. Pimodivir 600 mg twice daily for 5 days has been selected and is undergoing further evaluation in phase III clinical trials. in UniProtKB/Swiss-Prot.

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Pimodivir 600 mg twice daily for 5 days has been selected and is undergoing further evaluation in phase III clinical trials. Later, the influence of PB2-627K on the outcome of challenged mice was confirmed in H5N1 (25). However, almost none are known that target the PB2 replication protein, except for inducing its degradation. i want to know the difference about these two produce (NBP2-42878 and NBP2-42879). McCauley, W.S. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. Morbidity was assessed by body weight changes over a 14-day period post-inoculation. In addition, participates in the inhibition of type I interferon induction through interaction with and inhibition of the host mitochondrial antiviral signaling protein MAVS. Owen White 2020-09-17T19:36:33+00:00. Background Influenza A virus has a RNA-dependent RNA polymerase (RdRp) that is composed of three subunits (PB1, PB2 and PA subunit), which assemble with nucleoproteins (NP) and a viral RNA (vRNA) to form a RNP complex in the host nucleus. The present invention relates to soluble fragments of the Influenza virus RNA dependent RNA polymerase subunit PB2 and variants thereof, and crystallized complexes thereof comprising an RNA cap analog. Besides, the substitution of PB2-T271A enhanced the viral polymerase activity in mammalian cells.Conclusions The pathogenicity and replication of EA H1N1 virus containing 271A in … PB2-E627K is the best characterized human-adaptation mutation in AI viruses, particularly H5N1 viruses [31, 32], and is readily acquired by H9N2 viruses during passage in mice [33, 34]. Pimodivir is a non-nucleoside analogue that targets the cap-binding activity of the influenza A virus PB2 polymerase subunit. Systems used to automatically annotate proteins with high accuracy: Select one of the options below to target your search: Select item(s) and click on "Add to basket" to create your own collection here (400 entries max),

Manual validated information which has been generated by the UniProtKB automatic annotation system.

AB - Pimodivir (VX-787, JNJ-63623872) is an investigational anti-influenza drug, currently undergoing clinical development. The 1918 PB2 protein was found to be both necessary and sufficient for airborne transmission of a virus expressing the 1918 HA and neuraminidase. It is also a substrate for P-glycoprotein and OATP1B1 and also inhibits P-glycoprotein. Bake.

It should be noted that while, in theory, two different sequences could Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans. Recipes. PB2 Dsm, pPolI-PB2 A(TC), pPolI-PB2 Asm, pPolI-PB2 DAsm, and pPolI-PB2-S1 were generated by primer-based site-directed mutagenesis based on pPolI-PB2 (28). PB2 is the cap 1-recognition protein, which binds to the cap structure of host mRNA. The cap structure then acts as a primer for transcription to produce viral mRNA. Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))

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Manually curated information which has been propagated from a related experimentally characterized protein.

IMPORTANCEThe frequent reassortment among different influenza viruses … Influenza A Virus PB2 antibody detects PB2 protein at by immunofluorescent analysis.

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

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Sequence statusi: Complete.

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